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The 2 protein bands from forty kDa to thirty kDa ended up absent right after 24 h incubation at 45 °C. For the rest of the bands, various concentrations of all three proteases retained protein bands corresponding to their primary molar masses (Determine 6a,b). The existence of bands at minimal molecular bodyweight in fish roe just before hydrolysis was unpredicted. From the report by Ghelichi et al. [eight], slight peaks of a relatively low molecular weight vary (three–14 kDa) have been observed in defatted carp roe homogenate, potentially due to an autolytic system transpiring in fish roe and/or even the defatting procedure 23hoki bringing about the development of minimal-molecular-excess weight peptides.